Edesign  Primer and enhanced internal probe design tool

Paste source sequence below (5'->3', string of ACGTNacgtn -- other letters treated as N -- numbers and blanks ignored).
FASTA format ok. Please N-out undesirable sequence (vector, ALUs, LINEs, etc.) or use a Mispriming Library (repeat library)

Primer Pair
Pick Left (Forward) primer
Set Left primer as Eprimer
Use my own Left primer
Pick Right (Reverse) primer
Set Right primer as Eprimer
Use my own Right primer

Internal Probe
Pick Internal Probe
     Internal Probe Direction
     Set Internal Probe as Eprobe
     Use my own Internal Probe
     Genotyping by Internal Probe

Number To Return

Open All Conditions   Close All Conditions

Target Sequence Conditions (open)

Upload the settings from a file

Primer Picking Conditions (open)

Internal Probe Conditions (open)

General Conditions (open)

Open Penalty Weight Settings  Close Penalty Weight Settings