ECHO/DNA Thermodynamics

Please enter oligonucleotide sequence below.
- ECHO with thiazole orange doubly labeled thymidine (described as Z (T^E in the paper below)) only.
- Different lines are treated as different oligonucleodies.
- ECHO and Reverse complementary DNA sequences must be the same size.
- Mis-match base pair one-base next to Z(T^E) can not be calculated properly yet.
ECHO Sequences (using Z for T^E) (5'→3') :		Reverse Complement DNA Sequences (5'→3') :

Temperature: °C
[Na⁺] = M (typical PCR: 0.05M, typical SmartAmp: 0.01 M)
[Mg⁺⁺] = M (typical PCR: 0.0015M, typical SmartAmp: 0.008 M)
[ECHO] = [DNA] = μM

Users of the ECHO/DNA Thermodynamics page are requested to cite:

Kimura,Y., Hanami,T., Tanaka,Y., de Hoon,M.J.L., Soma,T., Harbers,M., Lezhava,A., Hayashizaki,Y. and Usui,K. Effect of Thiazole Orange Doubly Labeled Thymidine on DNA Duplex Formation. Biochemistry, 51, 6056-6067 (2012).